These microorganisms are important to humans because they play a role in the ecology of life, by decomposing wastes, both natural and man made, such as creating nitrogen fertilizer at the root zones of certain crops. Other several pathogens that can cause serious harm, even immediate death due to the diseases or disease causing products they produce. The purpose of this study was to identify the unknown bacterium using biochemical tests and various methods that had been learned from previous the microbiology laboratory class. Identifying the unknown bacterium was determined by separating and differentiating possible bacteria based on specific biochemical characteristics. The differential tests used to identify the unknown cultures were gram staining, oxidase, indole test, urea test, and casein test.

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Materials and methods: the unknown bacteria were given out by the lab instructor. All methods have been practiced to ensure proper procedure identifying bacteria have been applied to this unknown. Procedures were followed as stated in the course laboratory manual provided by the instructor, unless otherwise noted. Each test performed identified was used to determine the specifics and identify the unknown bacterium. The first procedure that needed to be accomplished was to streak the unknown out on a trypticase soy agar plate, using the t streak method described in the lab manual. The unknown culture was inoculated in the tryptic soy broth tube, a tsa plate agar, and a tsa slant.

Source normalized impact per paper snip: 1.216 ℹ source normalized impact per paper snip: 2014: 1.216 snip measures contextual citation impact by weighting citations based on the total number of citations in a subject field. Scimago journal rank sjr: 0.815 ℹ scimago journal rank sjr: 2014: 0.815 sjr is a prestige metric based on the idea that not all citations are the same. Sjr uses a similar algorithm as the google page rank it provides a quantitative and a qualitative measure of the journal’s impact. Impact factor: 2.561 ℹ impact factor: 2014: 2.561 the impact factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years.

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© thomson reuters journal citation reports 2015 5 year impact factor: 2.474 ℹ five year impact factor: 2014: 2.474 to calculate the five year impact factor, citations are counted in 2014 to the previous five years and divided by the source items published in the previous five years. © journal citation reports 2015, published by thomson reuters unknown lab report introduction there are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that i have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium. The purpose of this lab was to identify two unknown bacteria cultures using various differential tests.

The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were each person in the class was given a heterogeneous broth mixture of 2 microbes. This mixture was streaked onto the following plates: tsa, bap, mac, cna some of which are selective for gram + or gram microbes, while others aren’t specific.

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Isolation onto a bap would allow for a pure culture of just gram + which would be used to carry out the rest of the differential test. Isolation onto tsa/ mac allowed for the growth of gram and would be used for further test. Procedure: lab12 medical microbiology part1– differential media/biochemical tests, sp2012 set all of your margins to ½ purpose: the purpose of this lab is to help you become a little familiar with some of the tests that can be typically performed in a clinical or research lab facility. Preparing for class day 1 read in your lab manual about the following agar mediums: blood agar pg 168 , emb agar pg 170 , mannitol salt agar msa pg 172 , macconkey agar pg 174 , and pea agar pg 176 to answer the following: 1. What does the blood agar select for? blood agar allows distinction among bacteria based on their ability to lyse red blood cells hemolytic activity. What are the 3 types of blood agar results and how can you recognize them? beta hemolysis, which is the complete lysis of red blood cells and hemoglobin.

No hemolysis, sometimes called gamma hemolysis results in no change in the medium. What color is the emb agar? dark blue colonies with green metallic sheen or pink. How is it recognized? it contains the dyes eosin and methylene blue, which inhibit the growth of gram positive bacteria.

What color is the mannitol salt agar msa ? yellow color change in surrounding media. What does the msa agar select for? it contains 7.5% sodium chloride, which selects for organisms that are halotolerant. Which bacteria that we have learned about in lecture can be seen on msa agar? the organism staphylococcus aureus. How can it be recognized? capable of mannitol fermentation, signified by the color change in the surrounding media. We will be setting up the selective/differential media plates for each group as follows: each group will have one bacteria sample and will do streak plates on each of the 3 agar plates emb, pea and macconkey. Take care with your technique because each group’s plates will be shared with every group in the class.